Fig. 2

UCX® stimulate endothelial cell migration. Human umbilical vein endothelial cells (HUVECs) were seeded to confluence in endothelial basal medium (EBM) supplemented with 5 % FBS and cultured for 16 hours after wounding. Immediately after wound, HUVECs were co-cultured with UCX® or not (control) for 9 hours. (a) Representative images are shown. The left and right panels concern HUVEC monolayer immediately after or 9 hours post-wounding, respectively. (b) The wound area was measured immediately after and 9 hours post-wounding by using ImageJ software (three independent measurements were performed for each experimental group; t (2.03) = 4.66; *p < 0.05); effect size was 3.82 and power 0.93)