Fig. 3

UCX® increase perfusion recovery. UCX® or their vehicle (as a control) were administered in the ischemic gastrocnemius muscle 5 hours after HLI induction. (a) Representative laser doppler flow images before (PRE-HLI), immediately after (d0 POST-HLI) and at 7, 14 and 21 days post-HLI induction (d7 POST-HLI, d14 POST-HLI, d21 POST-HLI). (b) Quantitative evaluation of blood flow expressed as a ratio of ISC to NISC limb demonstrated significantly enhanced limb blood perfusion in UCX®-treated mice at 7, 14 and 21 days post-HLI. (n = 16 for each experimental group; D7: t (22.69) = 4.26; ***p < 0.001; effect size was 1.51 and power 0.98; D14: t (30) = 4.7; ***p < 0.001; effect size was 1.66 and power 0.99; D21: t (30) = 7.22; ***p < 0.001; effect size was 2.56 and power 0.99). HLI hindlimb ischemia, ISC ischemic, NISC non-ischemic