Fig. 3

Effects of hADSCs on the epithelialization and granulation tissue regeneration at D15 after treatment. a H&E staining of sections showed better dermal re-epithelialization on the foot ulcers in model rats treated with hADSCs compared with control model rats. Scale bar, 100 μm. b Granulation tissues in the hADSC-treated group were thicker than those in the PBS-treated group. Scale bar, 100 μm. c Statistical thickness of granulation tissues by computer-assisted morphometric analysis. d Collagen deposition assessed by Masson Trichrome staining. The hADSC-treated group showed more intense blue staining than the PBS-treated group, suggesting that hADSC treatment accelerated collagen deposition in the granulation tissues. Scale bar, 50 μm. e Quantification analysis of the Masson Trichrome staining section by digital image analysis. Averaged data presented as mean ± SD. *P < 0.05. hADSC human adipose-derived stem cell, PBS phosphate-buffered saline