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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Ex-vivo expansion of nonhuman primate CD34+ cells by stem cell factor Sall4B

Fig. 4

Determination of optimal MOI for GFP-Sall4B lentivirus in primate CD34+ cells. Nonhuman primate BM CD34+ cells transduced with GFP-control and GFP-Sall4B lentivirus at MOI of 0, 5, 20, 80, or 200 for 12 hours, and cultured for 9 days. a On day 9, total cell number was counted and CD34+ proportions were obtained by flow cytometry. The fold expansion of total nucleated cells and CD34+ cells was calculated based on the cell number on both day 0 and day 9. Data presented as mean ± SD. ## P < 0.01, vs Sall4B at MOI = 0 group (total cell); **P < 0.01, ***P < 0.001, vs Sall4B at MOI = 0 group (CD34+ cell). One-way ANOVA followed by Dunnett’s multiple comparison test. b Sall4B polyclonal antibody was used to detect Sall4B protein in cell lysates from all groups on day 9. β-actin was used as the loading control. Sall4B protein bands were detected at 76 kDa in a dose-dependent manner, with MOI at 5, 20, 80, or 200. GFP green fluorescent protein, MOI multiplicity of infection

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