Fig. 5

Expansion and characterization of Sall4B-transduced primate CD34⁺ cells. a Bright-field and fluorescent images of nonhuman primate BM CD34⁺ cells transduced with GFP-Sall4B lentivirus (i and ii, 20 ×) or GFP-control lentivirus (iii and iv, 20 ×) on day 9. The GFP-control CD34⁺ primate cell clusters were distinguishable from GFP-Sall4B lentivirus groups. b Flow cytometry measured the proportion of GFP-positive cells transduced with GFP-Sall4B or GFP-control lentivirus. c CD34⁺ percentage measured by flow cytometry on day 9 after transduction. **P < 0.01, GFP-Sall4B group vs GFP-control group. d On day 9, total nucleated cells were counted and CD34⁺ cells were calculated from the total cell number and measured CD34⁺ percentage. ***P < 0.001, GFP-Sall4B group vs GFP-control group. e CFU colonies formed from CD34⁺ cells transduced with GFP-Sall4B lentivirus or GFP-control lentivirus on day 9. Freshly purified CD34⁺ cells from day 0 were used as the positive control. The CFU colonies were counted on day 16 after cells were cultured in CFU MethoCult Media. **P < 0.01, vs freshly purified CD34⁺ cells from day 0. One-way ANOVA followed by Dunnett’s multiple comparison test. GFP green fluorescent protein, w/o without, BFU-E erythroid burst-forming unit, CFU-GEMM granulocyte–erythrocyte–macrophage–megakaryocyte colony-forming unit; CFU-GM granulocyte–monocyte colony-forming unit