Skip to main content
Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Mesenchymal stem cells improve mouse non-heart-beating liver graft survival by inhibiting Kupffer cell apoptosis via TLR4-ERK1/2-Fas/FasL-caspase3 pathway regulation

Fig. 2

Th1/Th2 responses and Kupffer cells are impaired after non-heart-beating (NHB) liver transplantation. At 6 h post-transplantation, liver grafts from each group were collected for quantitative RT-PCR analysis and immunofluorescence staining. Intrahepatic mRNA levels of a Th1 cytokines (TNFα, IFNγ, and IL2) and b Th2 cytokines (IL4, IL6, and IL10) in each group were measured by quantitative RT-PCR (N = 8–10 for each group). c Intrahepatic mRNA level of F4/80 was detected by quantitative RT-PCR (N = 8–10 for each group). d F4/80 immunofluorescence staining of frozen liver graft tissues were visualized by confocal scanning. Representative images from one experiment are shown at 600× magnification. e Intrahepatic F4/80-positive cells in each sections were counted for statistical analysis (N = 6 for each group). a, b, c, e Results are presented as mean ± SEM for each group. *P < 0.05, **P < 0.01, ***P < 0.001. IFN interferon, IL interleukin, TNF tumour necrosis factor

Back to article page