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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Composite cell sheet for periodontal regeneration: crosstalk between different types of MSCs in cell sheet facilitates complex periodontal-like tissue regeneration

Fig. 2

The crosstalk between hPDLSCs and hJBMMSCs in vitro. a, b Osteogenic differentiation/ALP activity and their quantitative results of co-cultured hPDLSCs/hJBMMSCs and hPDLSCs/hJBMMSCs by alizarin red staining and ALP staining. c, d The results of Western blot and quantitation show the expression of osteoblast- and ECM-related proteins in co-cultured hPDLSCs/hJBMMSCs and hPDLSCs/hJBMMSCs. e, f The results of PCR show the expression of osteoblast- and ECM-related genes in co-cultured hPDLSCs/hJBMMSCs and hPDLSCs/hJBMMSCs. P hPDLSCs, Co-P co-cultured hPDLSCs, B hJBMMSCs, Co-B co-cultured hJBMMSCs. The data are shown as mean ± SD. * P < 0.05, n = 3. The scale bar represents 50 μm

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