Fig. 4From: Composite cell sheet for periodontal regeneration: crosstalk between different types of MSCs in cell sheet facilitates complex periodontal-like tissue regenerationMorphological characteristics and immunohistochemical analyses of PDLSCS, JBMMSCS, and CSCS. a The arrangement of cells in all three cell sheets was observed by SEM. b H&E staining showed all three cell sheets were dense and contained plenty of cells, which could ensure collagen secretion. c-j All three cell sheets positively expressed ALP, BSP, COL-1, OCN, RUNX2, fibronectin, periostin and integrinβ1. ALP, BSP, OCN, RUNX2 and periostin indicated the osteogenic differentiation capacity. COL-1, integrinβ1, and fibronectin were the markers of ECM. PDLSCS periodontal ligament stem cell sheet, JBMMSCS jaw bone marrow-derived mesenchymal stem cell sheet, CSCS composite stem cell sheet. The scale bar represents 100 μmBack to article page