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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Composite cell sheet for periodontal regeneration: crosstalk between different types of MSCs in cell sheet facilitates complex periodontal-like tissue regeneration

Fig. 6

Regeneration of PDL/cementum-like tissue around hTDM in nude mice. a-d Typical arranged tissue with Sharpey-like fibers was regenerated, which was closely attached to the surface of hTDM at an oblique or horizontal angle in the CSCS group by H&E and Masson tricolor staining. e-h Few inserted fibers were observed, and most of fibers were parallel with the surface of the scaffolds in the PDLSCS group. i-l A few collagenous fibers, sparsely and chaotically arranged, in regenerated tissues in the JBMMSCS group. m, n Immunohistochemical staining showed high expression of CEMP1 and BSP in the CSCS group, especially in the juncture of the soft and hard tissues. o, p The expression of CEMP1 was positive, while the expression of BSP was negative in the PDLSCS group. q, r The low expressions of CEMP1 and BSP were observed in the JBMMSCS group. CLT cementum-like tissue, hTDM human treated dentin matrix, RF regenerated fibers. PDLSCS periodontal ligament stem cell sheet, JBMMSCS jaw bone marrow-derived mesenchymal stem cell sheet, CSCS composite stem cell sheet, hTDM human treated dentin matrix. The scale bar represents 100 μm

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