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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Heterogeneity of proangiogenic features in mesenchymal stem cells derived from bone marrow, adipose tissue, umbilical cord, and placenta

Fig. 3

Conditioned medium (CM) of BMSCs, AMSCs, UMSCs, and PMSCs exerts various proangiogenic effects on endothelial cells in vitro. CMs were generated from 106 cells after 48 hours of incubation with EBM2. CMs supplemented with 2 % FBS, EBM2 supplemented with 2 % FBS (negative control), and EGM2-MV (containing 2 % FBS, positive control) were used to culture endothelial cells and further comparatively analyze their effects on endothelial cell proliferation. a Similar to EGM2-MV, BMSCCM and PMSCCM significantly promoted endothelial cell proliferation in comparison with EBM2 after 48 hours of culture; however, AMSCCM and UMSCCM did not produce such a promotion effect. Each sample was performed in triplicate (n = 3, *p < 0.05, **p < 0.01). To determine the bioactivity of secreted factors from MSCs on endothelial cells, an in-vitro Matrigel tube formation assay was performed. Representative photographs were taken after 9 hours of incubation (scale bar = 500 μm). b Number of tube structures (c) was counted; total tube area (d) and total tube length (e) in each well were measured and analyzed using ImageJ software. Each sample was performed in duplicate (n = 3, *p < 0.05, **p < 0.01, ***p < 0.001). MSC mesenchymal stem cells, BMSC bone marrow-derived MSCs, AMSC adipose tissue-derived MSCs, UMSC umbilical cord-derived MSCs, PMSC placental chorionic villi-derived MSCs

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