Fig. 5

P311 promoted TGFβ1 promoter methylation and UTR activity. a Diagram of the CpG island in the mouse TGFβ1 proximal promoter ranging from –544 to +990 relative to the transcription start site. Vertical bars, CpG site; thick horizontal bar, CpG island. b DNA methylation status of the CpG island was analyzed in P311-transfected and control vector-transfected mouse EpSCs using bisulfite sequencing PCR. Solid circles, methylated cytosine residues; empty circles, unmethylated cytosine residues. c pmirGL0 UTR luciferase reporter constructs. d Luciferase reporter analysis of the TGFβ1 5′ and 3′ UTRs in mouse EpSCs that were transfected with a P311 adenovirus or an empty control vector (n = 3 per group). e TGFβ1 mRNA degradation curves. Actinomycin D was added to mouse EpSCs that were transfected with P311 or an empty control vector. The amount of TGFβ1 mRNA was normalized to the initial value at 0 hours (n = 4 per group). Data shown as mean ± SEM, Student t test, *P < 0.05,**P<0.01. UTR untranslated region