Fig. 2

Distribution of SSEA-4-expressing cells in adult human pancreatic exocrine cells. a CA19-9 expression in cultured exocrine cells (68.21 ± 6.57 %). b Amylase expression in cultured exocrine cells (12.22 ± 5.18 %) c Distribution of total cultured exocrine cells plotted according to cell size (common cell size: P2; small cell size: P3). d Negative isotype controls were analyzed using PE-conjugated IgG and FITC-conjugated IgG. e Distributions of SSEA-4 expressing cells in exocrine cells (Q1: 21.57 ± 3.17 %). f Correlation analysis of CA19-9 and SSEA-4 expression in exocrine cells: SSEA-4⁺ cells (Q1: 3.02 ± 1.73 %), SSEA-4⁺/CA19-9⁺ cells (Q2: 9.65 ± 2.68 %), SSEA-4^–/CA19-9^– cells (Q3: 10.92 ± 2.47 %), and SSEA-4^–/CA19-9⁺ cells (Q4: 71.35 ± 11.58 %). g Correlation analysis of amylase and SSEA-4 expression in exocrine cells in common cell size gating: SSEA-4⁺ cells (Q1: 18.20 ± 3.12 %), SSEA-4⁺/amylase-positive cells (Q2: 1.07 ± 0.58 %), SSEA-4^–/amylase-negative cells (Q3: 80.85 ± 6.56 %), and SSEA-4^–/amylase-positive cells (Q4: 10.75 ± 4.235). h Negative isotype controls in small cell size gating. i Distribution of SSEA-4-expressing cells (Q1: 12.44 ± 3.45 %). j Correlation analysis of CA19-9 and SSEA-4 expression in small-sized exocrine cells: SSEA-4⁺ cells (Q1: 1.97 ± 0.82 %), SSEA-4⁺/CA19-9⁺ cells (Q2: 7.72 ± 0.86 %), SSEA-4^–/CA19-9^– cells (Q3: 66.20 ± 7.84 %), and SSEA-4^–/CA19-9⁺ cells (Q4: 23.17 ± 25.76 %). k Correlation analysis of amylase and SSEA-4 expression in small cell size gating: SSEA-4⁺ cells (Q1: 7.32 ± 0.83 %), SSEA-4⁺/amylase-positive cells (Q2: 0.67 ± 0.36 %), SSEA-4^–/amylase-negative cells (Q3: 90.07 ± 4.22 %), and SSEA-4^–/amylase-positive cells (Q4: 5.12 % ± 2.59 %). IgG-PE phycoerythrin, SSEA-4 stage-specific embryonic antigen 4