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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Adult human pancreas-derived cells expressing stage-specific embryonic antigen 4 differentiate into Sox9-expressing and Ngn3-expressing pancreatic ducts in vivo

Fig. 3

Suspension cultures of adult human pancreas exocrine cells and separation for collection of pure SSEA-4+ cells. a Processes used for collection of pure single cells. Isolated crude exocrine cells from human partial pancreas tissues formed spheres during suspension culture. Exocrine cell spheres were separated into single cells by TE, and specific positive/negative cells were selected by MACS. b Spherical morphology of exocrine cells during suspension culture. c CA19-9 expression in spherical exocrine cells. b SSEA-4 expression in spherical exocrine cells. e Insulin expression in spherical exocrine cells. f SSEA-4 expression in cultured SSEA-4+ and SSEA-4 cells. g CA19-9 expression in cultured CA19-9+ and CA19-9 cells. h Viability of selected pure single SSEA-4+ cells or CA19-9+ cells during culture. Scale bars: 100 μm. Ab antibody, MACS magnetic-activated cell sorting, SSEA-4 stage-specific embryonic antigen 4

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