Fig. 4

Relative expression of key transcription factors as markers of ES cells. a Oct4, Nanog, Klf4, Sox2, and c-Myc expression in crude exocrine cells on days 2, 4, 6, and 8 of culture by conventional PCR. b Expression levels of Oct4, Nanog, Klf4, Sox2, and c-Myc in CA19-9+ and CA19-9– cells by qPCR. c Relative expression of Oct4, Nanog, Klf4, Sox2, and c-Myc in SSEA-4+ and SSEA-4– cells by qPCR. d Expression of Oct4, Nanog, Klf4, Sox2, and c-Myc in SSEA-4+ and CA19-9+ cells by qPCR. d days, SSEA-4 stage-specific embryonic antigen 4