Fig. 6

Expression of Sox9 and Ngn3 in newly formed pancreatic ductal cells. a Sox9 expression in the nuclei of newly formed pancreas ductal cells from tissues derived from SSEA-4⁺ cells. Scale bars: left panel, 50 μm; right panel, 10 μm. b Sox9 expression in newly formed pancreas ductal cells from tissues derived from CA19-9⁺ cells. Scale bars: left panel, 50 μm; right panel, 10 μm. c Percentage of Sox9⁺ cells in total pancreatic ductal cells from tissues derived from SSEA-4⁺ or CA19-9⁺ cells. **P = 0.0005. d Ngn3 expression in tissues derived from SSEA-4⁺ cells. Scale bars: left panel, 50 μm; right panel, 10 μm. e Ngn3 expression in tissues derived from CA19-9⁺ cells. Scale bars: left panel, 50 μm; right panel, 10 μm. f Percentage of Ngn3⁺ cells in total pancreatic ductal cells in tissues derived from SSEA-4⁺ or CA19-9⁺ cells. g Pdx1 expression in tissues derived from SSEA-4⁺ cells. h Pdx1 expression in tissues derived from CA19-9⁺ cells. i Ki-67 expression in newly formed pancreatic ductal cells from tissues derived from SSEA-4⁺ cells. j Ki-67 expression in newly formed pancreatic ductal cells from tissues derived from CA19-9⁺ cells. k Clusterin expression in newly formed pancreatic ductal cells from tissues derived from SSEA-4⁺ cells. l Clusterin expression in newly formed pancreatic ductal cells from tissues derived from CA19-9⁺ cells. Scale bars: g–l, 50 μm. SSEA-4 stage-specific embryonic antigen 4