Fig. 3

Wide expression of different autophagy components in independent iPSC lines. Proteins were extracted from iPSCs with daily renewal of culture medium. Then 15 μg of protein was loaded onto each lane. Lanes a–l represent 12 independent iPSC lines from 10 donors (1, 33D6; 2, JOM; 3, LV1; 4, LV2; 5, LV3; 6, 001CC1; 7, NRXN1C1; 8, 002 V; 9, 003 V; 10, SC126; 11, SC128; 12, SC132). (a–c) Immunoblotting was carried out with antibodies against LC3B-I, LC3B-II, BECLIN-1, AMPKα, ULK1, ULK2, ATG3, ATG12, ATG13, ATG101, and β-actin. (d–g) The relative abundance of the proteins was quantified using ImageJ software against β-actin and data were presented as mean ± SD. (h) Immunoblots were carried out to compare expression of ATG5 and ATG12 among three fibroblast lines (3× Fib.) and five iPSC lines (5× iPSCs) of healthy donors, showing higher ATG5 and ATG12 expression in iPSCs than that in fibroblasts. iPSC induced pluripotent stem cell