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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Rapamycin regulates autophagy and cell adhesion in induced pluripotent stem cells

Fig. 4

Rapamycin induces autophagy in time-dependent and concentration-dependent manners in iPSCs. iPSCs were maintained in six-well plates and medium was renewed daily. Images of LC3B staining were taken at 4 days of rapamycin treatment at 0, 10, 100, or 200 nM respectively (ad) and for 200 nM of rapamycin treatment at 1, 3, and 6 days respectively (eh), showing concentration-dependent and time-dependent induction. (i) Proteins were extracted from iPSCs treated with rapamycin at 0, 1, 10, 100, 200, or 300 nM for 4 days, and immunoblotted with antibodies against LC3B, phosphorylated ULK1, phosphorylated p70S6K, or β-actin. (jl) Relative abundance of the LC3B-II/I ratios (j), p-p70S6K (k), and p-ULK1 (l) was quantified using ImageJ software against a loading control β-actin. Data were presented as mean ± SEM, *p < 0.05, **p <0.01. Bar = 10 μm ah

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