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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Rapamycin regulates autophagy and cell adhesion in induced pluripotent stem cells

Fig. 7

Rapamycin induces spontaneous formation of uniform sizes of EBs. (ac) Images of floating spheres appeared after 9 days of rapamycin treatment (200 nM) from three iPSC lines. (df) Images of EBs made after 9 days from the cut-and-paste method. (g) EB area sizes (μm2) were quantified with ImageJ software and data were presented as mean ± SD. The X-axis in panel (g): 6f, 7a/b, 7c, 7d, 7e, 7f represents figure number, from which the EB sizes were quantified. Note the round shape and uniform sizes of EBs from rapamycin treatment (ac), in contrast to irregular shape and sizes of EBs resulted from the same iPSC lines by mechanical method (df). Bar = 200 μm. EB embryoid body

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