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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Defined three-dimensional culture conditions mediate efficient induction of definitive endoderm lineage from human umbilical cord Wharton’s jelly mesenchymal stem cells

Fig. 1

Experimental protocol and 3D colony formation. a Schematic representation of the differentiation protocol including the key manipulated signaling pathways. b Phase-contrast representative microscope images (Magnification x 200) for WJ-MSCs cultured in TC plate, AggreWell, and suspension. At days 3–7, cells formed floating clusters in suspension, whereas the control cells were detached and released from generated clusters

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