Skip to main content
Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Heme oxygenase-1-transduced bone marrow mesenchymal stem cells in reducing acute rejection and improving small bowel transplantation outcomes in rats

Fig. 1

Characterization of Ad/HO-1/BMMSCs in vitro and detection of their HO-1 expression. (a) Morphology of Ad/HO-1/BMMSCs with a bright field. BMMSCs exhibit spindle-shaped morphology and are arranged in whorls when transduced with Ad/HO-1. (b) Osteogenic differentiation of Ad/HO-1/BMMSCs in vitro by Von Kossa staining. Black deposition of calcium salt can be seen in positive cells. (c) Adipogenic differentiation of Ad/HO-1/BMMSCs in vitro by Oil Red O staining. Red lipid droplet formation can be seen in positive cells. Surface markers detection of Ad/HO-1/BMMSCs demonstrated that the proportion of CD29-positive and CD34-negative cells was >97% (d), the proportion of CD90-positive and CD45-negative cells was >95% (e), and the proportion of RT1A-positive and RT1B-negative cells was >96% (f). Immunocytochemical staining revealed that the red fluorescence intensity (HO-1 expression level) in the cytoplasm was higher in the Ad/HO-1/BMMSC-treated group (h) than in the BMMSC-treated group (g). (i) Western blot analysis results showed significantly higher HO-1 expression in Ad/HO-1/BMMSCs than in BMMSCs (*p < 0.05). Ad/HO-1 adenoviral vector carrying the heme oxygenase gene, BMMSC bone marrow mesenchymal stem cell, HO-1 heme oxygenase-1

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512