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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: MicroRNA-29 facilitates transplantation of bone marrow-derived mesenchymal stem cells to alleviate pelvic floor dysfunction by repressing elastin

Fig. 2

miR-29a-3p directly targets 3′-UTR of elastin mRNA. a Sequence alignment of miR-29a-3p with potential targeting site in the 3′-UTR of elastin mRNA. b Wild-type (-Luc-ELN-WT) or mutated (Luc-eln-mut) targeting sequences from elastin mRNA 3′-UTR were fused at the 3′ of the luciferase reporter open reading frame (Luc ORF). c Luciferase activities of Luc-ELN-WT and Luc-eln-mut constructs were measured in BMSCs transfected with negative control miR (miR-NC) or miR-29a-3p. d and e Expressions levels of elastin mRNA (d) and protein (e) were examined in BMSCs transfected with miR-NC or miR-29a-3p. Values were mean ± SD from three independent experiments. ** P < 0.01, ns not significant vs miR-NC

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