Fig. 5

miR-29a-3p inhibition synergized with bFGF-PLGA in promoting the expression and secretion of elastin in differentiated BMSCs. BMSCs transduced with negative control inhibitor (control) or miR-29a-3p-specific inhibitor were differentiated in the presence of either bFGF or bFGF-PLGA for 7 days. Levels of elastin mRNA (a), protein (b), and secreted elastin in medium (c) were examined by RT-PCR, Western blot, and ELISA assays, respectively. Values were mean ± SD from three independent experiments. ^* P < 0.05, ^** P < 0.01, ns not significant, vs BMSC (control) + bFGF. ^## P < 0.01 vs BMSC (control) + bFGF-PLGA and BMSC (anti-miR-29) + bFGF