Fig. 3
Hypoxia induces actin rearrangement and stress fibre formation in ECFCs. The ECFCs were grown in 12% FCS-supplemented EBM-2 medium and maintained in either 21 or 1% O2 for 24 h. ECFCs were stained with phallodin to identify filamentous actin (F-actin, green), vincullin (red) to identify focal adhesions and DAPI to stain the nuclei (blue). i, iv 40 × magnification, ii, v 60 × magnification. i, ii, iv, v Scale bars: 50 μm size marker. ii, v Magnified area depicted in iii and vi. Actin stress fibres (green) and focal adhesions (red) are evident in a single cell grown in 1% O2 (iii, vi). iii, vi Scale bar: 25 μm size marker (n = 3)