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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Hypoxia-induced responses by endothelial colony-forming cells are modulated by placental growth factor

Fig. 6

Hypoxia exposure decreases ECFC angiogenesis in vitro. a ECFCs were stained with PKH membrane dye and grown in Matrigel®, and the formation of tube-like structures was assessed after 48 h. Representative images for 21 and 1% O2 are shown. Scale bar: 100 μm. b Total tube area (μm2) was quantified using NIS Elements software (Nikon) for ECFCs grown in 21% O2 (empty bars) or 1% O2 (filled bars). Data plotted as mean ± SD. *p < 0.05. Scale bars: 100 μm (n = 3)

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