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Fig. 9 | Stem Cell Research & Therapy

Fig. 9

From: Hypoxia-induced responses by endothelial colony-forming cells are modulated by placental growth factor

Fig. 9

Anti-PlGF neutralising antibody blocks DMOG-induced inhibition of ECFC tube formation. ECFCs were stained with calcein (Invitrogen) and grown on Matrigel®. Cells were treated with conditioned medium harvested from ECFCs treated with a DMSO (vehicle) plus isotype control IgG, b DMOG plus isotype control IgG, c DMSO plus anti-PlGF neutralising antibody or d DMOG plus anti-PlGF neutralising antibody. e At 48 h, total tube area (μm2) was quantified using NIS Elements software (Nikon). Data plotted as mean ± SD and are representative of three independent experiments. **p < 0.01; ***p < 0.001. Scale bars: 200 μm (n = 3). DMOG dimethyloxalylglycine, PlGF placental growth factor

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