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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Phenotypic correction of Fanconi anemia cells in the murine bone marrow after carrier cell mediated delivery of lentiviral vector

Fig. 3

In situ gene delivery in FANCC –/– murine model. a Measurement of 2° TD by FACS between mT/mG HSPC as carrier cells and CD45.1 HSPCs as recipient cells (n = 3, **p < 0.001) b 1° and 2° transduced colony forming units (CFUs) expressing the corresponding florescent proteins. c Schematic diagram of in situ gene delivery model for FANCC correction and expansion of corrected cells. d Chimerism of the recipient FANCC–/– CD45.2 cells up to 3 months post-transplant (n = 4) e GFP-FANCC expression in the recipient (CD45.2) cells up to 3 months post-transplant indicating 2° TD events. Irradiation to induce selection pressure by DNA repair was given at weeks 6 and 11. Con control, GFP green fluorescent protein, HSPC hematopoietic stem and progenitor cell, KO knockout, MMC mitomycin-C, PBC peripheral blood cells, Ra irradiation

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