Fig. 1

Sodium nitroprusside (SNP) increases the frequency of LSK-CD34⁺ hematopoietic stem cells (HSCs). Murine lineage-negative (Lin ⁻) bone marrow cells were exposed to 100 μM of SNP for 3 days and the output cells were enumerated and analyzed by flow cytometry. a Absolute numbers of hematopoietic cells and lineage-negative cells generated in the cultures are depicted. b Frequency of LSK HSCs in the output cells. c Frequency of CD34⁻ and CD34⁺ HSCs in the gated LSK population is illustrated. d Graph shows absolute numbers of LSK, LSK-CD34⁻, and LSK-CD34⁺ HSCs. e The frequency of EdU⁺ cells in the gated LSK-CD34⁻ and LSK-CD34⁺ population is illustrated. The data are represented as mean ± SEM. N = 3. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. Also see Additional file 3 (Figure S1). NS not significant