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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Nitric oxide has contrasting age-dependent effects on the functionality of murine hematopoietic stem cells

Fig. 1

Sodium nitroprusside (SNP) increases the frequency of LSK-CD34+ hematopoietic stem cells (HSCs). Murine lineage-negative (Lin ) bone marrow cells were exposed to 100 μM of SNP for 3 days and the output cells were enumerated and analyzed by flow cytometry. a Absolute numbers of hematopoietic cells and lineage-negative cells generated in the cultures are depicted. b Frequency of LSK HSCs in the output cells. c Frequency of CD34 and CD34+ HSCs in the gated LSK population is illustrated. d Graph shows absolute numbers of LSK, LSK-CD34, and LSK-CD34+ HSCs. e The frequency of EdU+ cells in the gated LSK-CD34 and LSK-CD34+ population is illustrated. The data are represented as mean ± SEM. N = 3. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. Also see Additional file 3 (Figure S1). NS not significant

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