Fig. 2

Sodium nitroprusside (SNP) upregulates surface expression and mRNA of CD34 in the LSK-CD34⁻ HSCs. (a-e) Sort-purified LSK-CD34⁻ HSCs were exposed to various concentrations of SNP for 12 h. The cells were analyzed for the surface expression of CD34 by flow cytometry and for Cd34 mRNA expression by qRT-PCR. a Frequency of LSK-CD34⁺ cells formed in response to various concentrations of SNP. b, c The graph and the flow panel show mean fluorescence intensity (MFI) of CD34 fluorescence. d Dose-dependent effect of SNP on Cd34-specific mRNA levels in the treated cells. e The amounts of nitrites formed in the conditioned medium of cells. (f-j) Sort-purified LSK-CD34⁻ cells were exposed to 200 μM SNP for various time periods. The cells were analyzed for the surface expression of CD34 by flow cytometry. f The time-dependent increase in the frequency of LSK-CD34⁺ cells in response to SNP treatment is shown. g, h The graph and the flow panel show MFI of CD34 fluorescence. i Amounts of nitrites formed in the conditioned medium of the cells. j Expression of Cd34-specific mRNA in the cells treated with 200 μM SNP for 12 h. The data are represented as mean ± SEM. N = 3. **p ≤ 0.01, ***p ≤ 0.001. Also see Additional file 4 (Figure S2)