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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Nitric oxide has contrasting age-dependent effects on the functionality of murine hematopoietic stem cells

Fig. 3

Addition of the nitric oxide scavenger c-PTIO abolishes sodium nitroprusside (SNP)-mediated upregulation of CD34 expression. Sort-purified LSK-CD34 cells were exposed to 200 μM SNP for 12 h with or without the addition of 100 μM c-PTIO. The cells were analyzed for CD34 surface expression by flow cytometry, and Cd34-specific mRNA was quantified by qRT-PCR. a Graph shows frequency of LSK-CD34+ cells formed in cultures. b, c The graph and the flow panel show the mean fluorescence intensity (MFI) of CD34 fluorescence. d Relative expression of Cd34-specific mRNA in the treated cells. The data are represented as mean ± SEM. N = 3. ***p ≤ 0.001. Also see Additional file 5 (Figure S3). NS not significant

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