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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Nitric oxide has contrasting age-dependent effects on the functionality of murine hematopoietic stem cells

Fig. 4

Upregulation of CD34 expression by sodium nitroprusside (SNP) is dependent on de novo mRNA and protein synthesis. Sort-purified LSK-CD34 cells were treated with 200 μM SNP for 12 h with or without the addition of 5 μg/ml actinomycin D (Act D). The cells were subjected to qRT-PCR and flow cytometry analyses. a Relative expression of Cd34-specific mRNA in the treated cells. b The frequency of LSK-CD34+ cells formed in the cultures. c Effect of ActD on the mean fluorescence intensity (MFI) of CD34 fluorescence is depicted. d Sort-purified LSK-CD34 cells were treated with 200 μM SNP for 12 h with or without the addition of pruromycin (20 μg/ml). The cells were subjected to qRT-PCR analyses. Relative expression of Cd34-specific mRNA in the treated cells is illustrated. The data are represented as mean ± SEM. N = 3. ***p ≤ 0.001. NS not significant

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