Fig. 6
From: Human fetal and adult epicardial-derived cells: a novel model to study their activation
Human fetal EPDCs are more prone to undergo EMT. a Upon removal of SB from culture medium, adult EPDCs remained their epithelial character while fetal EPDCs underwent EMT. This was evident by the change in morphology, decrease in nuclear WT1 and increase in αSMA staining (scale bars: 100 μm; staining intensity was corrected to their corresponding SB control). b An EMT-PCR array was performed for SB, non- (empty) and TGFβ -stimulated EPDCs and visualized as clustergram using Ward’s linkage. Upon hierarchical clustering, unstimulated adult EPDCs (empty) were closely related to their SB control, while unstimulated fetal EPDCs clustered together with their TGFβ-treated condition. EPDCs epicardial-derived cells, SB medium containing SB431542, TGFβ transforming growth factor beta