Fig. 1
Scheme of the experimental setup. SVF harvested from a single donor was initially seeded out and cultured until reaching confluency (P0). The ASCs were at this point sorted out into a control group (CFCS) or four subpopulations (SP1–4) based on a forward- vs. side-scatter (FSC/SSC) gate or patterns of CD markers co-expression, respectively. All sorted populations were subsequently analyzed for immunophenotype at passages P0, P2, and P3, and adipo-, osteo-, and chondrogenic differentiation, clonogenic potential, and wound healing capacity at passage P2. The entire experiment was performed twice independently. Abbreviations: SVF stromal vascular fraction, C control, SP subpopulation, FSC forward scatter, CFU colony-forming unit