Fig. 3
Evolution of immunophenotype after sorting according to specific co-expression patterns. The subpopulations with different combinations of six CD markers (SP1–4) and the control population sorted on the forward scatter (CFSC) were cultured and analyzed at the end of the next two passages (P2 and P3). The expression levels are plotted against the 488-SSC as contour plots to visualize progression in expression patterns. P0 represents analysis of the cell suspension just prior to sorting, and the red lines represent cutoff values for sorting gates as determined from FMOs. Abbreviations: FMO fluorescence minus one