Fig. 5
Stem cell properties of the phenotypically selected subpopulations SP1–4 and the control subpopulation CFSC sorted on the forward scatter. a Histochemical analysis of the trilineage differentiation using oil red O, alizarin red, and alcian blue for adipo-, osteo-, and chondrogenesis, respectively. The insets depict fourfold zoom from the large field micrographs. The scale bar denotes 100 μm. b Transcriptional expression of differentiation regulators by real-time RT-PCR from two independent experiments carried out in triplicate (n = 6). c The colony-forming capacity was assessed after staining with crystal violet from two independent experiments carried out in replicate (n = 16). The data are presented as mean + SEM