Recovery of NHSCs from UCB tissue varies widely from donor to donor, but CD146 expression is a valuable marker for predicting successful isolation of UCB-NHSCs. a Results of performing a general linear regression, using the independent variables shown, to predict the successful isolation of NHSCs from UCB. Although >50 UCB samples were evaluated, only 30 were analyzed for all of the listed independent variables; in the case of CD31 and CD90, only 29 and 25 UCB samples, respectively, were analyzed. CD146 was the only marker in the original MNC population capable of predicting the successful isolation of UCB-NHSCs (p < 0.02). b Cell surface phenotype (% positive cells) was highly variable in the MNC populations. More than 50 UCB samples were collected and analyzed. UCB samples were not analyzed for all cell surface markers, resulting in an unequal number of data points. The median is shown for each marker. c The percent of CD146-positive cells in the initial MNC population is a valuable marker for predicting successful isolation of NHSCs from UCB. There is a 50% likelihood of obtaining NHSCs when the initial population contains 4.33% CD146+ cells.