Fig. 6

Extracellular matrix (ECM)-adherent umbilical cord blood-derived non hematopoietic stem cells (UCB-NHSCs) display the capacity for adipogenesis, myogenesis, osteogenesis, and angiogenesis in vitro. To examine differentiation capacity, UCB-NHSCs were seeded onto ECM or uncoated TCP plates and cultured to confluence. At confluence, the cultures were switched to differentiation media. a UCB cells cultured on ECM in adipocyte differentiation media formed Oil Red O-positive droplets. b UCB cells cultured on ECM in myogenic media formed myotubes containing multi-nucleated cells on the surface of the fibers. c UCB cells, cultured on ECM in regular growth media (open bars) or cardiomyogenic media (solid bars), expressed troponin T type 2 (TNNT2) and displayed enhanced expression in induction media. Similarly cultured human bone-marrow-derived mesenchymal stem cells (hBM-MSCs) displayed lower levels of TNNT2 expression compared to UCB cells, but also showed increased expression with induction media. d UCB cells, seeded at two different seeding densities (SD), were grown in osteogenic media on ECM and tissue culture plastic (TCP) culture surfaces. Von Kossa-positive mineral deposits were only observed with culture on ECM. e UCB cells cultured on Matrigel-coated wells developed capillary-like structures faster (48 h) than endothelial progenitor cells (positive controls, data not shown)