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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: RETRACTED ARTICLE: microRNA -140-5p inhibits colorectal cancer invasion and metastasis by targeting ADAMTS5 and IGFBP5

Fig. 2

ADAMTS5 and IGFBP5 are downregulated by miR-140 in CRC cells. a The mRNAs of ADAMTS5 and IGFBP5 contain putative binding sites of miR-140. b HCT116 and RKO cells were transfected with miR-140 mimic using oligofectamine. Oligofectamine alone (Control) and negative miRNA (NC) were the negative controls. The relative expression of miR-140 was determined by qRT-PCR after normalization to the internal control, RNU6B. *P < 0.05. c Protein expressions of ADAMTS5 and IGFBP5 were analyzed by Western blot. CRC cells transfected with siRNA specific against ADAMTS5 (siADAMTS5) and IGFBP5 (siIGFBP5), respectively, were the positive control. The quantitative results of Western blot were analyzed by Gel-Pro Analyzer 4.0 software, and GAPDH was used as a loading control. *P < 0.05. d ADAMTS5 and IGFBP5 mRNA levels were measured by qRT-PCR and GAPDH was used as the internal control. *P < 0.05. e HCT116 and RKO cells were transfected with miR-140 inhibitor (Anti-miR-140) using lipofectamine 2000. Lipofectamine 2000 (Control), scrambled miRNA inhibitor (Anti-NC) and co-transfection of miR-140 inhibitor and siRNA against ADAMTS5 (140i + siADAMTS5) or IGFBP5 (140i + siIGFBP5) were the negative controls. ADAMTS5 and IGFBP5 proteins were quantified by Western blot. *P < 0.05

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