Table 1 Primer set for each gene

CD44

Forward: 5′-AAGGTGGAGCAAACACAACC-3′

Reverse: 5′-AACTGCAATGCAAACTGCAAG-3′

CD166

Forward: 5′-TAGCAGGAATGCAACTGTGG-3′

Reverse: 5′-CGCAGACATAGTTTCCAGCA-3′

KLF4

Forward: 5′-CCCAATTACCCATCCTTCCT-3′

Reverse: 5′-ACGATCGTCTTCCCCTCTTT-3′

Nanog

Forward: 5′-GATTTGTGGGCCTGAAGAAA-3′

Reverse: 5′-CAGATCCATGGAGGAAGGAA-3′

OCT4

Forward: 5′-AGTGAGAGGCAACCTGGAGA-3′

Reverse: 5′-GCCGGTTACAGAACCACACT-3′

SOX2

Forward: 5′-AACCCCAAGATGCACAACTC-3′

reverse: 5′-GCTTAGCCTCGTGGATGAAC-3′

c-Myc

Forward: 5′-GGTGCTCCATGAGGAGACA-3′

Reverse: 5′-CCTGCCTCTTTTCCACAGAA-3′

EGFR

Forward: 5′-CTTTCGATACCCAGGACCAAG-3′

Reverse: 5′-CAACTTCCCAAAATGTGCCC-3′

β-catenin

Forward: 5′-TGGATGGGCTGCCTCCAGGTGAC-3′

Reverse: 5′-ACCAGCCCACCCCTCGAGCCC-3′

β-actin

Forward: 5′-TCCTTCCTGGGCATGGAG-3′

Reverse: 5′-AGGAGGGGCAATGATCTT-3′