Skip to main content
Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Molecular control of nitric oxide synthesis through eNOS and caveolin-1 interaction regulates osteogenic differentiation of adipose-derived stem cells by modulation of Wnt/β-catenin signaling

Fig. 1

Immunofluorescence localization of eNOS, CAV-1F92A, and CAV-1WT in lentiviral transduced eASCs and un-transduced cells. a Un-transduced eASCs show no endogenous endothelial nitric oxide synthase (eNOS) expression. b eNOS-transduced eASCs (green) show strong cytoplasmic expression. c Un-transduced eASCs show endogenous caveolin expression (red). d Wild-type caveolin-1 (CAV-1 WT)-transduced eASCs show significantly stronger expression. When e eNOS, f mutated caveolin-1 (CAV-1 F92A), and g CAV-1WT were transduced to eASCs using lentiviral vectors, eNOS showed cytoplasm localization (red) whereas both the CAV-1F92A and CAV-1WT showed plasma membrane localization (green). h Confocal microscopy analysis of co-transduction of eASCs with eNOS and CAV-1F92A resulted in cytoplasmic eNOS expression (red) and membrane localization of CAV-1F92A (green)

Back to article page