Fig. 5

Activity of a Runx2 reporter during eASC osteogenic differentiation. a Schematic of the Runx2.Hsp68-eGFP lentiviral reporter used to transduce eASCs. The construct contains 343 bp of Runx2 enhancer and Hsp68 minimal promoter upstream to the enhanced green fluorescent protein (eGFP). b Runx2 reporter containing eASCs were transduced with endothelial nitric oxide synthase (eNOS), eNOS + mutated caveolin-1 (eNOS + CAV-1 ^F92A), and eNOS + wild-type caveolin-1 (eNOS + CAV-1 ^WT) following osteogenic induction up to 11 days, and GFP signals were detected by fluorescence microscopy and c increased numbers of osteogenic nodules were observed. *p < 0.05, versus eNOS, eNOS + CAV-1^WT, eASC (osteogenic induction), and eASC (growth medium). DMEM Dulbecco’s modified Eagle’s medium