Fig. 6

Nitric oxide signaling modulates Wnt signaling in eASCs. Relative mRNA transcript analysis by qPCR shows that endothelial nitric oxide synthase (eNOS) and eNOS + mutated caveolin-1 (eNOS + CAV-1 ^F92A) transduced cells increased the expression of canonical Wnt ligands a Wnt3a and b Wnt8a, whilst downregulating c non-canonical Wnt5a. Relative mRNA transcripts analysis by qPCR shows that treatment with 2 mM l-N^G-nitroarginine methyl ester (l -NAME) downregulated d Wnt 3a and upregulated e Wnt5a expression. *p < 0.05  eNOS+CAV-1WT, CAV-1WT, CAV-1F92A, eASC (osteogenic induction), eASC (growth medium). and ^# p < 0.05 versus eNOS + wild-type caveolin-1 (CAV-1 ^WT), CAV-1^WT, CAV-1^F92A, eASC (osteogenic induction), and eASC (in DMEM); ^## p < 0.05, versus eNOS (l-NAME) eASC (osteogenic induction) and eASC (growth medium)