Table 2 Primers used for reverse transcription quantitative polymerase chain reaction
Gene | Forward (5’ > 3’) | Reverse (5’ > 3’) | Accession number |
|---|---|---|---|
β-actin | ATGGATGATGATATCGCCGC | AGGTCTCAAACATGATCTGGG | NM_001081838 |
Runx2 | TCCACCACGCCGCTGTCT | TCAGTGAGGGATGAAATGCT | XM_005603968 |
ALP | TCATCGACATCTGGAAGAGC | GCTCAAAGAGACCCAAGAGG | XM_008537803 |
Wnt3a | TCAAGATCAGCATCCAGGAG | GTTGACAGTGGTGCAGTTCC | XM_014730421 |
Wnt8a | CCCAAGGCCTATCTGACCTA | AGCCTGTTGTGAGTGGACAG | XM_014730656 |
Wnt5a | CGAAGACAGGCATCAAAGAA | TATCTGCATAACCCTGCCAA | XM_014731495 |
GFP | AGCACTGCACGCCGTAGGTC | CGAGCTGGACGGCGACGTAA | KX349734 |