Fig. 4

Analysis of the morphology and adhesion properties of ADSCs from SSc patients. There was no alteration in the adhesive properties or the morphology of adipose-derived stem cells from systemic sclerosis patients (ADSC-SSc) compared to healthy control ADSCs (ADSC-N). a DNA quantification assay was carried out to determine the number of cells adhering to tissue culture plastic 24 h after seeding. Adhesion of ADSCs was assessed at passage 1 (P1) to P4. There was no statistical difference in the number of cells that adhered between ADSC-SSc and ADSC-N (n = 6). b Representative bright field images of ADSC-N at passage 2 (top left panel) and SSc-ADSC s at passage 2 (top right panel) (10× magnification). Representative images of Rhodamine Phalloidin staining of F-actin in ADSC-N at passage 2 (bottom left panel) and ADSC-SSc at passage 2 (bottom right panel) (40× magnification). Cells were counter-stained with DAPI to visualize DNA. Scale bars = 50 μm. c Cell area (left graph) and circularity index (right graph) of ADSC-SSc and ADSC-N at P1 to P4 was assessed 24 h after seeding using image J software (n = 20). Statistical significance was calculated using unpaired t test