Fig. 2
CG-induced SOX9 upregulation was associated with chondrogenic differentiation. a Expression of SOX5 and SOX6 mRNA in CG-stimulated ASCs. ASCs were centrifuged at 2400 g for 30 min and then harvested at the indicated time points. ** p < 0.01 for ASCs stimulated with CG versus the control (non-stimulated ASCs). Expression of SOX5 and SOX6 mRNA was analyzed using real-time PCR. Expression of (b) ACAN and COL2A1 mRNA, and (c) COL1 and COL10 mRNA, in ASCs stimulated with or without CG. For CG stimulation, ASCs (P2–3, 1 × 105 cells/mL) were centrifuged in a 15 mL tube at 2400 g for 30 min and then harvested after 24 h. Expression of corresponding genes was measured by reverse transcriptase PCR. ACAN aggrecan, CG centrifugal gravity, COL1 collagen type I, COL10 collagen type X, COL2A1 collagen type II ​alpha 1, TGF transforming growth factor