Fig. 1

Self-assembly methods for cartilage formation by human mesenchymal stem cells (hMSCs). a Top, disc culture: hMSCs were seeded in transwell inserts coated with vehicle control (Veh), type I collagen (Col1), or type II collagen (Col2); Veh group failed to form discs. Bottom, pellet culture: hMSCs were seeded in round-bottom wells and formed spherical aggregates. b Representative images of hyaline discs formed in coated transwells are shown