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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Extracellular matrix components and culture regimen selectively regulate cartilage formation by self-assembling human mesenchymal stem cells in vitro and in vivo

Fig. 3

Mechanical and physical properties of discs formed by self-assembling hMSCs. a Young’s modulus of engineered cartilage discs at day 42 measured by unconfined compressive loading. Type I collagen (Col1) discs outperformed type II collagen (Col2) discs. Increasing seeding density from 0.5 × 106 to 1.0 × 106 further improved Col1 discs. Only successfully formed discs were evaluated. b Disc thicknesses at day 42. Col1 and Col2 discs exhibited similar thicknesses; increasing seeding density increased the thickness of Col1 discs. c Representative stress–strain curves. d Comparison of the frequency of successful disc formation. Col1 coating enabled the highest yield of disc formation, but this effect was abolished at high seeding density (1.5 × 106 per well). Veh vehicle

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