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Table 1 Summary of studies on disc culture of self-assembling chondrocytes or MSCs for in vitro cartilage formation

From: Extracellular matrix components and culture regimen selectively regulate cartilage formation by self-assembling human mesenchymal stem cells in vitro and in vivo

Cell type Source Density (× 106/mm2) Insert type Substrate Disc formation (Yes/No) Thickness (mm) Reference
Chondrocytes Bovine MCC joint 0.050 Millicell PTFE Type II collagen Yes - [9]
Bovine MCC joint 0.016 Millicell PTFE Type II collagen Yes 0.3 [12]
Bovine MCC joint 0.160 - CPP Yes 0.94 [13]
Sheep MCC joint 0.160 - CPP Yes 0.5 [14]
Bovine hock joint 0.067 Millicell PTFE Type II collagen Yes 0.45 [15]
Bovine MCC joint 0.033 Millicell PTFE Type II collagen Yes - [16]
Bovine distal femur 0.172 - Agarose Yes 0.15 [17]
Bovine distal femur 0.289 - Agarose Yes 0.8 [18]
Human femoral cartilage 0.006 Millicell PTFE Type I/Type II collagen Yes 0.15 [19]
Human articular cartilage 0.102 - Agarose Yes 0.6 [20]
MSCs Human BM 0.015 Corning PC None Yes 0.8 [8]
Porcine BM 0.392 Millicell PET None Yes 1.3 [21]
Human BM 0.015 Corning PC None No - [11]
Sheep BM 0.033 Millicell PTFE Type IV collagen/CPP Yes 0.5 [22]
Equine cord blood 0.033 Millicell Fibronectin Yes 0.1 [23]
Human BM 0.102 - Agarose No - [20]
  1. In some cases, MSCs failed to form discs
  2. MSC mesenchymal stem cell, MCC convex condyle of the metacarpus, CPP calcium polyphosphate, BM bone marrow