Table 1 Human genes used in quantitative real-time PCR
From: Oxidative status predicts quality in human mesenchymal stem cells
Gene | Primer nucleotide sequence (5′ → 3′) | Amplicon (bp) |
|---|---|---|
Housekeeping gene | ||
GAPDH | F - TGGACTCCACGACGTACTCA | 102 |
R - GGAAGCTTGTCATCAATGGAA | ||
Mitochondrial biogenesis | ||
POLG | F - GCTGGTGGAAGAGCGTTACTC | 241 |
R - GAAGCTGCTTAGCCCTGAGAT | ||
POLG2 | F - GGTTTGGGGGTCGAGTAGATG | 186 |
R - TTCCACTTAGGAAATGCCTTCTC | ||
NRF1 | F - AACAAAATTGGGCCACGTTACA | 291 |
R - TCTGGACCAGGCCATTAGCA | ||
TFAM | F - ATGGCGTTTCTCCGAAGCAT | 133 |
R - CAGATGAAAACCACCTCGGTAA | ||
PGC-1α | F - GCTTTCTGGGTGGACTCAAGT | 142 |
R - TCTAGTGTCTCTGTGAGGACTG | ||
PGC-1β | F - CCACATCCTACCCAACATCAAG | 93 |
R - CACAAGGCCGTTGACTTTTAGA | ||
Mitochondrial chaperones | ||
PHB | F - CACAGAAGCGGTGGAAGC | 96 |
R - ATGGCCGCCTITTTCTGT | ||
PHB2 | F - TGCTGAACCTACAGGATGAAA | 77 |
R - TGGTGACTAGGCTCATITCTTACC | ||
MRP5S | F - GAAATCGATCCGTGTCTTGG | 87 |
R - GCATCCATCCGATCAGTAGC | ||
Telomere length | ||
Telomere | teloF - CGGTTTGTTTGGGTTTGGGTTTGGGTTTGGGTTTGGGTT | >76 |
teloR - GGCTTGCCTTACCCTTACCCTTACCCTTACCCTTACCCT | ||
36B4 | F - CAGCAAGTGGGAAGGTGTAATCC | 75 |
R - CCCATTCTATCATCAACGGGTACAA | ||
mtDNA copy number | ||
ND1 | F - GGAGTAATCCAGGTCGGT | 265 |
R - TGGGTACAATGAGGAGTAGG | ||
CF | F - AGCAGAGTACCTGAAACAGGAA | 482 |
R - AGCTTACCCATAGAGGAAACATAA | ||