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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Optimizing mesoderm progenitor selection and three-dimensional microniche culture allows highly efficient endothelial differentiation and ischemic tissue repair from human pluripotent stem cells

Fig. 1

Generation of MESP1+ cells from human embryonic stem cells. a Mesp1 progeny cells formed yolk sac blood vessels. Images of Mesp1 lineage cells (green) on the yolk sac of E9.5 embryos of Mesp1cre/+/ROSA26Sor tm4(ACTB-tdTomato,-EGFP)Luo genetic background. b Image showing MESP1-mTomato knock-in reporter cells. c Protocol to derive MESP1+ cells from hESCs by treatment with BMP4 and CHIR99021. d Percentage of MESP1+ cells on day 3 under different induction conditions (n = 3, **** p < 0.0001.). e FACS analysis showing, on day 3, that 97.93% of cells turned on MESP1-mTomato after treatment with BMP4 (25 ng/ml) and CHIR (10 μM). f Q-PCR analysis showing the downregulation of pluripotency, endoderm and ectoderm marker genes, and upregulation of mesoderm and cardiac markers genes in MESP1+ cells vs. MESP cells (n = 3). g MESP1-mTomato reporter expression co-localized with anti-MESP1 antibody (arrowheads). Scale bars: 100 μm

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