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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Optimizing mesoderm progenitor selection and three-dimensional microniche culture allows highly efficient endothelial differentiation and ischemic tissue repair from human pluripotent stem cells

Fig. 2

High-throughput RNA sequencing analysis of MESP1-mTomato mesoderm progenitor cells. a Flow chart of MESP1-mTomato cell gene expression analysis. b and c Genes upregulated and downregulated in MESP1-mTomato+ cells compared with hESCs (fold change > 1.5). They were divided into different groups based on their FPKM values in hESC, MESP1-mTomato+, and MESP1-mTomato cells. The number of genes in each group, the top GO term, and the enrichment p values are listed. d Q-PCR validation of key genes enriched in MESP1+ cells according to the RNA-seq result (n = 3, * p < 0.05, ** p < 0.005, *** p < 0.001, MESP1+ versus MESP1; t test)

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